RNA
Part:BBa_K1218000:Design
Designed by: Alex Constantino Group: iGEM13_Stanford-Brown (2013-08-16)
Minimal CRISPR Array
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 213
Illegal BsaI.rc site found at 190
Design Notes
Contains a leader sequence followed by repeat-spacer-repeat. Requires a trans-activating CRISPR RNA (tracrRNA) for post-transcriptional processing.
Source
Cloned from Streptococcus pyogenes gDNA by the Marraffini lab; subcloned by Stanford-Brown iGEM.