RNA

Part:BBa_K1218000:Design

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-16)


Minimal CRISPR Array


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213
    Illegal BsaI.rc site found at 190


Design Notes

Contains a leader sequence followed by repeat-spacer-repeat. Requires a trans-activating CRISPR RNA (tracrRNA) for post-transcriptional processing.


Source

Cloned from Streptococcus pyogenes gDNA by the Marraffini lab; subcloned by Stanford-Brown iGEM.

References